Composite

Part:BBa_K1769010

Designed by: Ho-Tsang Tsai   Group: iGEM15_NYMU-Taipei   (2015-09-18)

J61051+RBS+LuxR+Ter+pLUX+RBS+LuxI+Ter+pLux+RBS+Aiia+Ter+pLux+RBS+Ter

This is a template for us to add the reporter before the last terminator by Gibson assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
    Illegal BglII site found at 2938
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 3249
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2218
    Illegal BsaI.rc site found at 3087
    Illegal BsaI.rc site found at 4107


[edit]
Categories
Parameters
None